Conjugation guide

Whether it’s a western blot analysis or profiling the tumor microenvironment using multiplex imaging, we rely on antibodies conjugated to various labels to visualize and understand protein structure, function, localization, and other protein-protein interactions.

This comprehensive guide covers the ins and outs of antibody conjugation, including the difference between direct and indirect assays, common labels used, different chemistries behind antibody labeling, and methods you can use to conjugate your primary antibody.

• Antibody conjugation Antibody conjugation, also known as antibody labeling, is the process of linking an antibody to a specific tag or label.
• Direct vs indirect assays What's the difference between a direct and indirect assay?
• Antibody labeling chemistries Conjugation can be complex, and requires a basic understanding of chemical modification.
• Conjugation kits Conjugation kits simplify conjugation and are therefore an excellent option for those without chemical expertise.
• Confirming successful antibody conjugation Measuring the degree of labeling using a lateral flow assay can confirm successful antibody conjugation.